Which of the following is NOT a method for initiating the uptake of a plasmid into a target prokaryotic cell?
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Correct Answer: C. Lambdaphage viral vector.
The lambdaphage viral vector can be used injecting a specific sequence of DNA into a target cell, but not for encouraging the cell to uptake plasmid DNA. Bacteria can utilize a pillus for conjugation, in order to exchange genetic material through plasmids. Electroporation involves using a small charge to cause the cell membrane to become more porous and uptake a plasmid. Heat shock involves heating up the target cells to ~42ºC after keeping them on ice in the presence of CaCl2 – the cell membranes will become more porous and uptake the plasmid as with electroporation.
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