DNA polymerases catalyze the production of polynucleotide chains. Which of the following statements holds true:
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Correct Answer: D. DNA polymerases require a primer base-paired to the template and with a free 3′-end.
DNA polymerase can add dNTPs (deoxynucleoside triphosphates) only to a pre-existing oligonucleotide chain that is annealed to the template DNA. The enzyme adds dNTPs on a free 3′-OH- group. Primers in replication are RNA based. These primers are then cut out via endonuclease activity and replaced with DNA nucleotides. Finally, DNA ligase fuses the gap between the fragments of DNA.
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